Cannot Access The File Because It Is Being Used By Another Process - Using ASPNETDB.MDF

I'm using Visual Web Developer 2005 Express, SQL Server 2005 and using the local file system as opposed to IIS for development purposes.

I will reference two pages of my site:  one that manages Types of Tours and one that downloads a Tour Registration Excel file.

On the Tour Types page, there is a sqldatasource that populates a DetailsView whose default mode is Insert.  On that same page I have a GridView that is populated from the same sqldatasource. The gridview has Paging, Editing and Deleting enabled.  There is no code behind on this page except for a prompt to the user to confirm the delete action if he has elected to delete a Tour Type.  This page works fine no problems.

On the download page, I have a prompt that allows the user to click a button to download the Tour Registration Excel file.  The code behind opens the Registration table in the ASPNETDB.mdf database, builds an .xls file via a streamwriter and prompts the user to download the file.

My problem:  if I start up VWD, go into my project, go straight to the download page without accessing any other pages, the download page works fine.  If I access any other pages first and then go to the download page, I get the error "System.Data.SqlClient.SqlException: Unable to open the physical file "C:....App_DataASPNETDB.MDF". Operating system error 32: "32(The process cannot access the file because it is being used by another process."  My simplified code behind is this:

Dim sw As System.IO.StreamWriter
Dim myConn As Data.SqlClient.SqlConnection, query As String, myCommand As Data.SqlClient.SqlCommand
Dim myReader As Data.SqlClient.SqlDataReader
Dim fileName As String, baseDirectory As String, fullFileName As String

fullFileName = baseDirectory & fileName
sw = New IO.StreamWriter(fullFileName)

myConn = New Data.SqlClient.SqlConnection("Persist Security Info=False;Integrated Security=true;AttachDbFileName=C:<pathnamehere>App_DataASPNETDB.mdf;database=ASPNET;Data Source=.SQLEXPRESS")

query = "SELECT Registrations.RegistrationNum, Registrations.Title, Registrations.LastName, Registrations.FirstName, Registrations.Organization, Registrations.StreetAddr1, Registrations.StreetAddr2, Registrations.City, Registrations.State, Registrations.Zip, Registrations.WorkPhone, Registrations.HomePhone, Registrations.CellPhone,, Registrations.AddToMailingList, Registrations.NumberInParty, Registrations.Comments, Registrations.DateRegistered, Registrations.TourDate, Registrations.TourFormattedTime, TourTypes.TourTypeDescr FROM Registrations INNER JOIN TourTypes ON Registrations.TourTypeNum = TourTypes.TourTypeNum"

myCommand = New Data.SqlClient.SqlCommand(query, myConn)

As soon as the code his the myConn.Open(), it abends with the above message.  I then have to close the project, and stop and re-start SQL Server.  HELP!!

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ASPNETDB.MDF: The Process Cannot Access The File Because It Is Being Used By Another Process

I'm trying to upload the ASPNETDB.MDF file to a hosting server via FTP, and everytime when it was uploaded half way(40% or 50%)
I would get an error message saying:
"550 ASPNETDB.MDF: The process cannot access the file because it is being used by another process"
 and then the upload failed.
 I'm using SQL Express. Does anybody know what's the cause?
 Thanks a lot

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FCB::Open: Operating System Error 32(The Process Cannot Access The File Because It Is Being Used By Another Process.) Occurred W

Hello all,
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FCB:pen: Operating system error 32(The process cannot access the file because it is being used by another process.) occurred while creating or opening file 'C:Program FilesMicrosoft SQL ServerMSSQL.1MSSQLDataTest.mdf'. Diagnose and correct the operating system error, and retry the operation.
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FCB:pen failed: Could not open file C:Program FilesMicrosoft SQL ServerMSSQL.1MSSQLDataTest.mdf for file number 1. OS error: 32(The process cannot access the file because it is being used by another process.).

I've caught references to the auto close feature being a possible culprit, no dice as the databases in question are set to False. Recovery mode varies on the databases from Simple to Full. If I cycle the SQL Server service, whatever transient issue it was having with those files is gone.
As much as I'd love to disable the virus scanner, network security would not be amused. The data and log files appear to have the same permissions as unaffected database files. Nothing's set to read only or archive as I've caught on other forums as possible gremlins. I have sufficient disk space and the databases are set for unrestricted growth.

Any thoughts on what I could look at? If it was everything coming up in RECOVERY_PENDING it's make more sense to me than a hit or miss type of thing I'm experiencing now.

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Step 3
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Personally, I don't like using the Advanced log for job steps in this manner, but that's the way things are done around here. I'm trying to preserve this work flow with only minor tweaks, preferrably to the database server as a whole, but I'll do minor code changes if necessary.

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Why do I get this error? It appeared when I changed my PC for a new one that has Vista and therefore SP2. My BS2005 has been patched with the tow SP1 patches (SP1 for VS, SP1 for VS in Vista).

The reason I execute my package in BIDS is that we have a product made for SSIS and we want to demonstrate its use visually with SSIS. Any suggestion?

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